Microorganismos presentes en larvas de Comadia redtenbacheri Hammerschmidt (Lepidoptera: Cossidae).

Abstract

El objetivo del presente estudio fue aislar e identificar los microorganismos presentes en larvas de gusano rojo del maguey Comadia redtenbacheri Hammerschmidt (Lepidoptera: Cossidae), para mejorar técnicas de colecta y manejo de poblaciones silvestres, así como las del sistema de cría en condiciones de laboratorio. Se analizaron las bacterias de larvas sanas y enfermas tanto externas como del intestino de poblaciones, con y sin manejo durante la colecta y el acopio. El análisis filogenético del gen 16S rRNA reveló la existencia de los géneros y especies Paenibacillus sp., Bacillus safensis, Pseudomonas sp., Bacillus pseudomycoides, Corynebacterium variabile, Enterococcus sp., Gordonia sp., Acinetobacter calcoaceticus, Arthrobacter sp., Micrococcus sp. y Bacillus cereus. La mayor abundancia de estos organismos se encontró en poblaciones de larvas con manejo y dentro de ellas, las consideradas como enfermas. En pupas del insecto se aislaron hongos pertenecientes a los géneros Rhizopus sp., Penicillium sp. y Beauveria sp., identificados con mayor abundancia en tratamientos que se mantuvieron con humedad. _______________ MICROORGANISMS PRESENT IN Comadia redtenbacheri HAMMERSCHMIDT (LEPIDOPTERA: COSSIDAE) LARVAE. ABSTRACT: The objective of this study was to isolate and identify the microorganisms present in the larvae of the agave red worm Comadia redtenbacheri Hammerschmidt (Lepidoptera: Cossidae) to improve techniques for collection and management of wild populations as well as for the system of rearing in the laboratory. Bacteria from healthy and diseased larvae, both external and from intestines, with and without handling during collection and gathering, were analyzed. The phylogenetic analysis of the 16S rRNA gene revealed the existence of the following genera and species: Paenibacillus sp., Bacillus safensis, Pseudomonas sp., Bacillus pseudomycoides, Corynebacterium variabile, Enterococcus sp., Gordonia sp., Acinetobacter calcoaceticus, Arthrobacter sp., Micrococcus sp. and Bacillus cereus. The greatest abundance of these organisms was found in populations that had been handled and, of these, in specimens considered diseased. In pupae, fungi belonging to the genera Rhizopus sp., Penicillium sp. and Beauveria sp., were found and were more abundant in treatments maintained with moisture.